Your browser version may not work well with NCBI's Web applications. More information here...
1: EMBO J. 2007 Apr 4;26(7):1984-94. Epub 2007 Mar 15.
Related Articles, Links
Click here to read Click here to read Click here to read
Structural framework for DNA translocation via the viral portal protein.

Lebedev AA, Krause MH, Isidro AL, Vagin AA, Orlova EV, Turner J, Dodson EJ, Tavares P, Antson AA.

York Structural Biology Laboratory, Department of Chemistry, University of York, York, UK.

Tailed bacteriophages and herpesviruses load their capsids with DNA through a tunnel formed by the portal protein assembly. Here we describe the X-ray structure of the bacteriophage SPP1 portal protein in its isolated 13-subunit form and the pseudoatomic structure of a 12-subunit assembly. The first defines the DNA-interacting segments (tunnel loops) that pack tightly against each other forming the most constricted part of the tunnel; the second shows that the functional dodecameric state must induce variability in the loop positions. Structural observations together with geometrical constraints dictate that in the portal-DNA complex, the loops form an undulating belt that fits and tightly embraces the helical DNA, suggesting that DNA translocation is accompanied by a 'mexican wave' of positional and conformational changes propagating sequentially along this belt.

Publication Types:
PMID: 17363899 [PubMed - indexed for MEDLINE]

PMCID: PMC1847669