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Sharad Kumar
Department of Haematology, Institute of Medical and Veterinary Science (IMVS), Adelaide, Australia

Faculty Member: Cell Biology > Cellular Death & Stress Responses [ since 4 July 2001 ]
[ Sponsorship ] [ Biography ] [ Evaluations ]
Sponsorship

Sharad Kumar sponsors free access to F1000 for Centro de Estudios Cientificos, Valdivia, Chile. Supporting F1000's initiative to make itself available to scientists in all countries, including free access to developing countries (more details).

Biography

Sharad Kumar is a Professor and Senior Principal Research Fellow at the Hanson Institute in Adelaide. He obtained his PhD from the University of Adelaide. Following this he worked at the University of Queensland (Brisbane, Australia), CSIRO Animal Health Laboratory (Geelong, Australia), RIKEN Tsukuba Life Science Centre (Tsukuba, Japan), and the Cancer Institute (Tokyo, Japan), before returning to Adelaide in mid 1994. While in Japan, he cloned a number of developmentally regulated gene (named Nedd1-Nedd10). Since that time, Sharad has been pursuing functional analyses of some of the Nedd gene. He currently heads the Molecular Regulation Laboratory at the Hanson Centre for Cancer Research. His main interests lie in studying various aspects of apoptosis and the regulation of ubiquitin-mediated protein turnover via the Nedd4 family of ubiquitin-protein ligases. He also has a keen interest in animal development and the use of functional genomics in understanding disease process.

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Evaluations

Caspase-8 deficiency facilitates cellular transformation in vitro.
Krelin Y, Zhang L, …, Kovalenko A, Wallach D
Cell Death Differ 2008 Sep 15(9):1350-5 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar
Evaluated 21 Aug 2008

Centrosome Dysfunction in Drosophila Neural Stem Cells Causes Tumors that Are Not due to Genome Instability.
Castellanos E, Dominguez P, Gonzalez C
Curr Biol 2008 Jul 23 [abstract on PubMed] [related articles] [full text]
Selected by | Sharad Kumar / Anna Akhmanova / Yukiko Yamashita / Kristin White / Marcos Gonzalez-Gaitan
Evaluated 31 Jul 2008

The ubiquitin ligase Nedd4-1 is dispensable for the regulation of PTEN stability and localization.
Fouladkou F, Landry T, …, Stambolic V, Rotin D
Proc Natl Acad Sci U S A 2008 Jun 18 [abstract on PubMed] [related articles] [full text]
Selected by | Sharad Kumar
Evaluated 24 Jun 2008

Salt-Sensitive Hypertension and Cardiac Hypertrophy in Mice Deficient in the Ubiquitin Ligase Nedd4-2.
Shi PP, Cao XR, …, Stokes JB, Yang B
Am J Physiol Renal Physiol 2008 Jun 4 [abstract on PubMed] [related articles] [full text]
Selected by | Sharad Kumar
Evaluated 10 Jun 2008

Chk1 suppresses a caspase-2 apoptotic response to DNA damage that bypasses p53, Bcl-2, and caspase-3.
Sidi S, Sanda T, …, D'Andrea AA, Look AT
Cell 2008 May 30 133(5):864-77 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | John Abrams / Sharad Kumar / Matthew O'Connell / Surender Kharbanda
Evaluated 6 Jun 2008

Activation mechanism and substrate specificity of the Drosophila initiator caspase DRONC.
Snipas SJ, Drag M, Stennicke HR, Salvesen GS
Cell Death Differ 2008 May 15(5):938-45 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar
Evaluated 7 May 2008

Distinct mechanisms of apoptosis-induced compensatory proliferation in proliferating and differentiating tissues in the Drosophila eye.
Fan Y, Bergmann A
Dev Cell 2008 Mar 14(3):399-410 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Eric Baehrecke / Sharad Kumar
Evaluated 20 Mar 2008

The mammalian SPD-2 ortholog Cep192 regulates centrosome biogenesis.
Zhu F, Lawo S, …, Hyman AA, Pelletier L
Curr Biol 2008 Jan 22 18(2):136-41 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / David Glover
Evaluated 24 Jan 2008

A nonapoptotic cell death process, entosis, that occurs by cell-in-cell invasion.
Overholtzer M, Mailleux AA, …, Cibas ES, Brugge JS
Cell 2007 Nov 30 131(5):966-79 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Andrew Lumsden / Eric Baehrecke / Jonathan Chernoff / Gary Bokoch
Evaluated 3 Dec 2007

The deubiquitinating enzyme UCH-L3 regulates the apical membrane recycling of the epithelial sodium channel.
Butterworth MB, Edinger RS, …, Johnson JP, Frizzell RA
J Biol Chem 2007 Dec 28 282(52):37885-93 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 5 Nov 2007

Global changes to the ubiquitin system in Huntington's disease.
Bennett EJ, Shaler TA, …, Schulman H, Kopito RR
Nature 2007 Aug 9 448(7154):704-8 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Allan Tobin / Ulrich Hartl / Sharad Kumar / Takashi Tsuruo
Evaluated 21 Aug 2007

Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo.
Takemoto K, Kuranaga E, …, Miyawaki A, Miura M
Proc Natl Acad Sci U S A 2007 Aug 14 104(33):13367-72 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar
Evaluated 13 Aug 2007

Voltage-dependent anion channels are dispensable for mitochondrial-dependent cell death.
Baines CP, Kaiser RA, …, Craigen WJ, Molkentin JD
Nat Cell Biol 2007 May 9(5):550-5 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Terrence Frey
Evaluated 16 Apr 2007

Drosophila Bcl-2 proteins participate in stress-induced apoptosis, but are not required for normal development.
Sevrioukov EA, Burr J, …, Song EJ, Brachmann CB
Genesis 2007 Apr 45(4):184-93 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Christoph Borner / Eric Baehrecke
Evaluated 13 Apr 2007

Programmed anuclear cell death delimits platelet life span.
Mason KD, Carpinelli MR, …, Huang DC, Kile BT
Cell 2007 Mar 23 128(6):1173-86 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Monica Justice / Christoph Borner
Evaluated 28 Mar 2007


Apoptosis initiated when BH3 ligands engage multiple Bcl-2 homologs, not Bax or Bak.
Willis SN, Fletcher JI, …, Adams JM, Huang DC
Science 2007 Feb 9 315(5813):856-9 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | John Abrams / Simon Cook / Girish Putcha / Sharad Kumar / Albert La Spada
Evaluated 28 Mar 2007

Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation.
Oliver PM, Cao X, …, Marrack P, Yang B
Immunity 2006 Dec 25(6):929-40 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar
Evaluated 9 Jan 2007

RNA interference screen reveals an essential role of Nedd4-2 in dopamine transporter ubiquitination and endocytosis.
Sorkina T, Miranda M, …, Zahniser NR, Sorkin A
J Neurosci 2006 Aug 2 26(31):8195-205 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 18 Aug 2006

The Drosophila caspase Ice is important for many apoptotic cell deaths and for spermatid individualization, a nonapoptotic process.
Muro I, Berry DL, …, Baehrecke EH, Hay BA
Development 2006 Sep 133(17):3305-15 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 18 Aug 2006

Cell survival through Trk neurotrophin receptors is differentially regulated by ubiquitination.
Arévalo JC, Waite J, …, Lee FS, Chao MV
Neuron 2006 May 18 50(4):549-59 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar
Evaluated 19 May 2006

The effector caspases drICE and dcp-1 have partially overlapping functions in the apoptotic pathway in Drosophila.
Xu D, Wang Y, …, Ding T, Bergmann A
Cell Death Differ 2006 Oct 13(10):1697-706 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 9 May 2006

Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice.
Hara T, Nakamura K, …, Okano H, Mizushima N
Nature 2006 Jun 15 441(7095):885-9 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Eric Baehrecke / Daniel Klionsky / Sharad Kumar / Nektarios Tavernarakis
Evaluated 9 May 2006


Loss of autophagy in the central nervous system causes neurodegeneration in mice.
Komatsu M, Waguri S, …, Kominami E, Tanaka K
Nature 2006 Jun 15 441(7095):880-4 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Ted Dawson / Eric Baehrecke / Daniel Klionsky / Sharad Kumar / Ulf Pettersson / Nektarios Tavernarakis / Thorsten Hoppe
Evaluated 9 May 2006


Caspase-9 holoenzyme is a specific and optimal procaspase-3 processing machine.
Yin Q, Park HH, …, Jiang X, Wu H
Mol Cell 2006 Apr 21 22(2):259-68 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar
Evaluated 5 May 2006

The apoptosome activates caspase-9 by dimerization.
Pop C, Timmer J, Sperandio S, Salvesen GS
Mol Cell 2006 Apr 21 22(2):269-75 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar
Evaluated 5 May 2006

Lipocalin 2-deficient mice exhibit increased sensitivity to Escherichia coli infection but not to ischemia-reperfusion injury.
Berger T, Togawa A, …, Cheung CC, Mak TW
Proc Natl Acad Sci U S A 2006 Feb 7 103(6):1834-9 [abstract on PubMed] [related articles] [FREE full text]
Selected by | David Vaux / Sharad Kumar
Evaluated 3 Feb 2006

Three-dimensional structure of a double apoptosome formed by the Drosophila Apaf-1 related killer.
Yu X, Wang L, …, Wang X, Akey CW
J Mol Biol 2006 Jan 20 355(3):577-89 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Yigong Shi
Evaluated 5 Dec 2005

Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway.
Kleino A, Valanne S, …, Lemaitre B, Rämet M
EMBO J 2005 Oct 5 24(19):3423-34 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 7 Oct 2005

An RNA interference screen identifies Inhibitor of Apoptosis Protein 2 as a regulator of innate immune signalling in Drosophila.
Gesellchen V, Kuttenkeuler D, …, Pelte N, Boutros M
EMBO Rep 2005 Oct 6(10):979-84 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 7 Oct 2005

Caspase-2 primes cancer cells for TRAIL-mediated apoptosis by processing procaspase-8.
Shin S, Lee Y, …, Choi H, Kim K
EMBO J 2005 Oct 19 24(20):3532-42 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 7 Oct 2005

Michelob_x is the missing inhibitor of apoptosis protein antagonist in mosquito genomes.
Zhou L, Jiang G, …, Severson DW, Xiao L
EMBO Rep 2005 Aug 6(8):769-74 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar
Evaluated 27 Jul 2005

ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor.
Chen D, Kon N, …, Qin J, Gu W
Cell 2005 Jul 1 121(7):1071-83 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Atan Gross
Evaluated 18 Jul 2005

Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis.
Zhong Q, Gao W, Du F, Wang X
Cell 2005 Jul 1 121(7):1085-95 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Atan Gross / Ding Xue
Evaluated 15 Jul 2005

Specific ablation of the apoptotic functions of cytochrome C reveals a differential requirement for cytochrome C and Apaf-1 in apoptosis.
Hao Z, Duncan GS, …, Wang X, Mak TW
Cell 2005 May 20 121(4):579-91 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Yigong Shi / Sharad Kumar / John Abrams / Astar Winoto
Evaluated 2 Jun 2005

The CARD-carrying caspase Dronc is essential for most, but not all, developmental cell death in Drosophila.
Xu D, Li Y, …, Lackey M, Bergmann A
Development 2005 May 132(9):2125-34 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Kristin White / Sharad Kumar
Evaluated 15 Apr 2005

Cyclophilin D-dependent mitochondrial permeability transition regulates some necrotic but not apoptotic cell death.
Nakagawa T, Shimizu S, …, Kubo T, Tsujimoto Y
Nature 2005 Mar 31 434(7033):652-8 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Eduardo Rial / Robert Sapolsky / Atan Gross
Evaluated 14 Apr 2005

Loss of cyclophilin D reveals a critical role for mitochondrial permeability transition in cell death.
Baines CP, Kaiser RA, …, Robbins J, Molkentin JD
Nature 2005 Mar 31 434(7033):658-62 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Eduardo Rial / Robert Sapolsky / Atan Gross
Evaluated 14 Apr 2005

Obligate role of anti-apoptotic MCL-1 in the survival of hematopoietic stem cells.
Opferman JT, Iwasaki H, …, Akashi K, Korsmeyer SJ
Science 2005 Feb 18 307(5712):1101-4 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar / Shiv Pillai / Gerald Spangrude
Evaluated 22 Feb 2005

Generation and characterization of endonuclease G null mice.
Irvine RA, Adachi N, …, Hsieh CL, Lieber MR
Mol Cell Biol 2005 Jan 25(1):294-302 [abstract on PubMed] [related articles] [FREE full text]
Selected by | David Vaux / Sharad Kumar
Evaluated 20 Dec 2004

Neuroprotective role of the Reaper-related serine protease HtrA2/Omi revealed by targeted deletion in mice.
Martins LM, Morrison A, …, Mak T, Downward J
Mol Cell Biol 2004 Nov 24(22):9848-62 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 2 Nov 2004

Caspase-8 serves both apoptotic and nonapoptotic roles.
Kang TB, Ben-Moshe T, …, Lapidot T, Wallach D
J Immunol 2004 Sep 1 173(5):2976-84 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Charles Czuprynski / Sharad Kumar
Evaluated 10 Sep 2004

cAMP and serum and glucocorticoid-inducible kinase (SGK) regulate the epithelial Na(+) channel through convergent phosphorylation of Nedd4-2.
Snyder PM, Olson DR, …, Zhou R, Steines JC
J Biol Chem 2004 Oct 29 279(44):45753-8 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 9 Sep 2004

Compensatory proliferation induced by cell death in the Drosophila wing disc requires activity of the apical cell death caspase Dronc in a nonapoptotic role.
Huh JR, Guo M, Hay BA
Curr Biol 2004 Jul 27 14(14):1262-6 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Ken Irvine
Evaluated 6 Aug 2004

Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity.
Xirodimas DP, Saville MK, …, Hay RT, Lane DP
Cell 2004 Jul 9 118(1):83-97 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Yunde Zhao / Sharad Kumar
Evaluated 26 Jul 2004

A role for Drosophila IAP1-mediated caspase inhibition in Rac-dependent cell migration.
Geisbrecht ER, Montell DJ
Cell 2004 Jul 9 118(1):111-25 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Ed Manser / Kristin White
Evaluated 15 Jul 2004

Caspase-2 can function upstream of bid cleavage in the TRAIL apoptosis pathway.
Wagner KW, Engels IH, Deveraux QL
J Biol Chem 2004 Aug 13 279(33):35047-52 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 18 Jun 2004

Regulation of an ATG7-beclin 1 program of autophagic cell death by caspase-8.
Yu L, Alva A, …, Baehrecke EH, Lenardo MJ
Science 2004 Jun 4 304(5676):1500-2 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar / Vojo Deretic
Evaluated 17 May 2004

The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress.
Tinel A, Tschopp J
Science 2004 May 7 304(5672):843-6 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar / Eui-Ju Choi / Surender Kharbanda
Evaluated 15 Apr 2004

A large-scale RNAi screen in human cells identifies new components of the p53 pathway.
Berns K, Hijmans EM, …, Beijersbergen RL, Bernards R
Nature 2004 Mar 25 428(6981):431-7 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / Julian Downward / William Kaelin
Evaluated 29 Mar 2004

Multiple apoptotic caspase cascades are required in nonapoptotic roles for Drosophila spermatid individualization.
Huh JR, Vernooy SY, …, Guo M, Hay BA
PLoS Biol 2004 Jan 2(1):E15 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Kristin White / Sharad Kumar
Evaluated 4 Mar 2004

Relative contribution of Nedd4 and Nedd4-2 to ENaC regulation in epithelia determined by RNA interference.
Snyder PM, Steines JC, Olson DR
J Biol Chem 2004 Feb 6 279(6):5042-6 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar
Evaluated 23 Jan 2004

Regulation of NF-kappaB-dependent lymphocyte activation and development by paracaspase.
Ruefli-Brasse AA, French DM, Dixit VM
Science 2003 Nov 28 302(5650):1581-4 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar / Sankar Ghosh
Evaluated 19 Nov 2003

Loss of Omi mitochondrial protease activity causes the neuromuscular disorder of mnd2 mutant mice.
Jones JM, Datta P, …, Meisler MH, Alnemri ES
Nature 2003 Oct 16 425(6959):721-7 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Sharad Kumar / M. Flint Beal / Michael Sendtner
Evaluated 20 Oct 2003

Suppression of CED-3-independent apoptosis by mitochondrial betaNAC in Caenorhabditis elegans.
Bloss TA, Witze ES, Rothman JH
Nature 2003 Aug 28 424(6952):1066-71 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Christoph Borner / Sally Kornbluth / Sharad Kumar / John Abrams
Evaluated 25 Sep 2003

The Drosophila Mst ortholog, hippo, restricts growth and cell proliferation and promotes apoptosis.
Harvey KF, Pfleger CM, Hariharan IK
Cell 2003 Aug 22 114(4):457-67 [abstract on PubMed] [related articles] [order article]
Selected by | Sharad Kumar
Evaluated 15 Sep 2003

Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes.
Micheau O, Tschopp J
Cell 2003 Jul 25 114(2):181-90 [abstract on PubMed] [related articles] [order article]
Selected by | Sharad Kumar / Mark Bothwell / Paul Lyons / Christoph Borner / Gourisankar Ghosh / Carl Ware
Evaluated 30 Jul 2003

The serine/threonine kinase Pim-2 is a transcriptionally regulated apoptotic inhibitor.
Fox CJ, Hammerman PS, …, Chodosh LA, Thompson CB
Genes Dev 2003 Aug 1 17(15):1841-54 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar / Andrew D. Sharrocks
Evaluated 24 Jul 2003

Elimination of Mcl-1 is required for the initiation of apoptosis following ultraviolet irradiation.
Nijhawan D, Fang M, …, Du F, Wang X
Genes Dev 2003 Jun 15 17(12):1475-86 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Sharad Kumar / Yigong Shi
Evaluated 9 Jun 2003

The Drosophila microRNA Mir-14 suppresses cell death and is required for normal fat metabolism.
Xu P, Vernooy SY, Guo M, Hay BA
Curr Biol 2003 Apr 29 13(9):790-5 [abstract on PubMed] [related articles] [order article]
Selected by | Sharad Kumar
Evaluated 16 May 2003

Caspase activity and a specific cytochrome C are required for sperm differentiation in Drosophila.
Arama E, Agapite J, Steller H
Dev Cell 2003 May 4(5):687-97 [abstract on PubMed] [related articles] [order article]
Selected by | Sharad Kumar / Kristin White
Evaluated 17 Apr 2003

Mechanism of XIAP-mediated inhibition of caspase-9.
Shiozaki EN, Chai J, …, Fairman R, Shi Y
Mol Cell 2003 Feb 11(2):519-27 [abstract on PubMed] [related articles] [order article]
Selected by | Sharad Kumar
Evaluated 7 Mar 2003

A unified model for apical caspase activation.
Boatright KM, Renatus M, …, Green DR, Salvesen GS
Mol Cell 2003 Feb 11(2):529-41 [abstract on PubMed] [related articles] [order article]
Selected by | Yigong Shi / Sharad Kumar / Christoph Borner / William Earnshaw
Evaluated 6 Mar 2003

Genome-wide analyses of steroid- and radiation-triggered programmed cell death in Drosophila.
Lee CY, Clough EA, …, Stephan DA, Baehrecke EH
Curr Biol 2003 Feb 18 13(4):350-7 [abstract on PubMed] [related articles] [order article]
Selected by | Sharad Kumar / Carl Thummel
Evaluated 27 Feb 2003